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Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed TCRβ chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® Rabbit mAb #55750). Original magnification 200× and 400×.

Journal: Biomedicines

Article Title: Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease

doi: 10.3390/biomedicines13102526

Figure Lengend Snippet: Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed TCRβ chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® Rabbit mAb #55750). Original magnification 200× and 400×.

Article Snippet: The primary antibodies that were used were the following: CD3 (clone LN10, Leica Biosystems, Leica K.K., Shinjuku-ku, Tokyo, Japan), CD4 (4B12, Leica), CD8 (4B11, Leica), CD103 (EP206, Leica), granzyme B (11F1, Leica), TCRβ (TRBC1/TCRβ constant region 1 (E6Z3S) Rabbit mAb #79485, Cell Signaling Technology K.K., Chiyoda-ku, Tokyo, Japan), TCRδ (TRDC/TCRδ (E2E9T) XP ® Rabbit mAb #55750, Cell Signaling), CD56 (CD56-504-L-CE, Leica), CD16 (CD16-L-CE, Leica), LAIR1 (CD305, JAVI82A, created by Giovanna Roncador, Spanish National Cancer Research Center (CNIO, Centro Nacional de Investigaciones Oncologicas, calle Melchor Fernandez Almagro, 3, 28029 Madrid, Spain), PD-L1 (73-10, Leica), PD1 (CD279, NAT105, CNIO), BTLA (CD272, FLO67B, CNIO), TOX2 (TOM924D, CNIO), HVEM (TNFRSF14, ab47677, Abcam Tokyo, Ota city, Tokyo, Japan), CD163 (CD163-L-CE, Leica), HLA-DP-DQ (JS76, CNIO), IL4I1 (BALI265E,543H,573B, CNIO), and FOXP3 (236A, CNIO).

Techniques: Control, Clone Assay

Main phenotype of IELs in control intestinal mucosa. This figure summarizes the main immunophenotypes of IELs, including CD3+, CD8+, CD103+, LAIR+, and TCRβ+. An area with marked aggregation of IELs and immune cells in the lamina propria is shown. Original magnification 400×.

Journal: Biomedicines

Article Title: Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease

doi: 10.3390/biomedicines13102526

Figure Lengend Snippet: Main phenotype of IELs in control intestinal mucosa. This figure summarizes the main immunophenotypes of IELs, including CD3+, CD8+, CD103+, LAIR+, and TCRβ+. An area with marked aggregation of IELs and immune cells in the lamina propria is shown. Original magnification 400×.

Article Snippet: The primary antibodies that were used were the following: CD3 (clone LN10, Leica Biosystems, Leica K.K., Shinjuku-ku, Tokyo, Japan), CD4 (4B12, Leica), CD8 (4B11, Leica), CD103 (EP206, Leica), granzyme B (11F1, Leica), TCRβ (TRBC1/TCRβ constant region 1 (E6Z3S) Rabbit mAb #79485, Cell Signaling Technology K.K., Chiyoda-ku, Tokyo, Japan), TCRδ (TRDC/TCRδ (E2E9T) XP ® Rabbit mAb #55750, Cell Signaling), CD56 (CD56-504-L-CE, Leica), CD16 (CD16-L-CE, Leica), LAIR1 (CD305, JAVI82A, created by Giovanna Roncador, Spanish National Cancer Research Center (CNIO, Centro Nacional de Investigaciones Oncologicas, calle Melchor Fernandez Almagro, 3, 28029 Madrid, Spain), PD-L1 (73-10, Leica), PD1 (CD279, NAT105, CNIO), BTLA (CD272, FLO67B, CNIO), TOX2 (TOM924D, CNIO), HVEM (TNFRSF14, ab47677, Abcam Tokyo, Ota city, Tokyo, Japan), CD163 (CD163-L-CE, Leica), HLA-DP-DQ (JS76, CNIO), IL4I1 (BALI265E,543H,573B, CNIO), and FOXP3 (236A, CNIO).

Techniques: Control